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has gloss | eng: Calcium chloride (CaCl2) transformation is a laboratory technique in cell biology. It increases the ability of a cell to incorporate plasmid DNA. Transformation can then occur. The addition of calcium chloride to a cell suspension promotes the binding of plasmid DNA to the cell surface, which can then pass into the cell. CaCl2 transformation is also accompanied by heat shock, where cells are cooled to a low temperature (~4 degrees Celsius) and then heated to a high temperature (~42 degrees Celsius) for a short time. |
lexicalization | eng: Calcium Chloride Transformation |
instance of | c/Molecular biology techniques |
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