e/Expanded bed adsorption

Information
has gloss	eng: Expanded bed adsorption (EBA) is a preparative chromatographic technique which makes processing of viscous and particulate liquids possible. Where classical column chromatography uses a solid phase made by a packed bed, EBA uses particles in a fluidized state. Expanded bed adsorption is, however, different from fluidised bed chormatography in essentially two ways: one, the EBA resin contains particles of varying size and density which results in a gradient of particle size when expanded; and two, when the bed is in its expanded state, local loops are formed. Particles such as whole cells or cell debris, which would clog a packed bed column, readily pass through a fluidized bed. EBA can therefore be used on crude culture broths or slurries of broken cells, thereby bypassing initial clearing steps such as centrifugation and filtration, which is mandatory when packed beds are used. The feed flow rate is kept low enough that the solid packing remains stratified and does not fluidize completely. Hence EBA can be modeled as frontal adsorption in a packed bed, rather than as a well mixed continuous-flow adsorber.
lexicalization	eng: Expanded bed adsorption
instance of	c/Biochemistry methods