| has gloss | eng: PET Applications #DNA-PET: Because PET represent connectivity between the tags, the use of PET in genome re-sequencing has advantages over the use of single reads. Anchoring one half of the pair uniquely to a single location in the genome allows mapping of the other half that is ambiguous. Ambiguous reads are those that map to more than a single location. This increased efficiency reduces the cost of sequencing as these ambiguous sequences, or reads, would normally be discarded. The connectivity of PET sequences also allows detection of structural variations: insertions, deletions, duplications, inversions, translocations. During the construction of the PET library, the fragments can be selected to all be of a certain size. After mapping, the PET sequences are thus expected to be consistently a particular distance away from each other. |
